Recombinant CD3 anticorps (AA 20-40)
Aperçu rapide pour Recombinant CD3 anticorps (AA 20-40) (ABIN7477391)
Antigène
Voir toutes CD3 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 20-40
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Immunogène
- A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)
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Isotype
- IgG
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anti-CD3 (CD3) antibody
CD3 Reactivité: Humain, Souris, Rat IHC, IF, WB Hôte: Lapin Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 Reactivité: Humain, Non-Human Primate FACS, IP, IHC (fro), ICC, MS Hôte: Souris Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 Reactivité: Humain FACS, IP, MS Hôte: Souris Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 Reactivité: Humain, Non-Human Primate FACS, IP, IHC (fro), Func, MS Hôte: Souris Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 Reactivité: Humain FACS, IP, Func, MS Hôte: Souris Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 Reactivité: Humain FACS, IP Hôte: Souris Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 Reactivité: Humain, Non-Human Primate FACS, IP, IHC (fro) Hôte: Souris Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (APC)CD3 Reactivité: Humain FACS Hôte: Souris Monoclonal MEM-57 APC
anti-CD3 (CD3) antibody (PE)CD3 Reactivité: Humain FACS Hôte: Souris Monoclonal MEM-57 PE
anti-CD3 (CD3) antibody (PerCP)CD3 Reactivité: Humain, Non-Human Primate FACS Hôte: Souris Monoclonal UCHT1 PerCP
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells).
Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD3
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Autre désignation
- CD3
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Sujet
- CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07766
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Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
Antigène
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